Ultrafast capillary electrophoresis and bioanalytical applications

Ultrafast capillary electrophoresis and bioanalytical applications.

T he advancement of our knowledge of biochemical phenomena depends on our ability to implement increasingly more powerful analytical techniques. Traditional means to characterize the chemical constituents of a solution include spectroscopic approaches and separation techniques to identify, isolate and quantify analytes. Unfortunately, these techniques are not perfect. Often, spectral properties...

CLINICAL APPLICATIONS Capillary Electrophoresis

Column Zorbax silica, 25 cm 4.6 mm internal diameter (7 m) S5W uncoated silica, 25 cm 4.6 mm internal diameter (5 m) Eluent Hexane}ethyl acetate, 95 : 5 CO2 modified with small amounts of methanol Flow rate 1.6 mL min 1 2 mL min 1 for 4 min, then gradient of 2 up to 5 mL min 1 thereafter held constant Programme Isocratic P"100 atm, T"403C; modifier, 1.5%min 1 from 10% to 30% thereafter held con...

Biomedical applications of capillary electrophoresis.

After having grown through the stages of technique development and instrumentation availability, capillary electrophoresis has reached the stage of applications. This review attempts to show the potential of this technique for biomedical analysis. Rather than going into a detailed description of the technical details of the separation conditions suitable for the separation of a particular categ...

Capillary Electrophoresis-Based Immunoassays: Principles &Quantitative Applications

The use of capillary electrophoresis as a tool to conduct immunoassays has been an area of increasing interest over the last decade. This approach combines the efficiency, small sample requirements, and relatively high speed of CE with the selectivity of antibodies as binding agents. This review examines the various assay formats and detection modes that have been reported for these assays, alo...

Introduction to Quantitative Applications of Capillary Electrophoresis in Pharmaceutical Analysis Introduction to Quantitative Applications of Capillary Electrophoresis in Pharmaceutical Analysis